In-situ hybridisation

In-situ hybridisation (ISH) is a molecular biological method for identifying DNA or RNA sequences in cells and tissues using a complementary sequence known as a probe. Its applications are many and varied and the main advantage of this method is that the detection of nucleic acid sequences can be directly linked to tissue morphology. 

Our comprehensive selection of different in-situ hybridisation techniques enable you to investigate gene expression or genome changes in your samples.

We offer customised in-situ hybridisation techniques for human and animal tissue (for FFPE tissue and frozen sections).

Our in-situ hybridisations are largely fully automated on our Leica BOND immunostainer. This way, we guarantee consistent, reproducible results of the highest quality and optimum preservation of the tissue morphology at the same time.

We would be pleased to support you with our many years of experience in the search for suitable probes and carry out optimisation on suitable positive and negative controls.

RNAscope™ ISH is a groundbreaking in situ hybridisation technology. ACD's patented probe design amplifies target-specific signals in situ, and thus enables the detection and quantification of an individual RNA molecule. Automated assays are available for chromogen and fluorescence detection and the latest detection kits also enable RNA-protein co-detection. Please contact us if you require further information on the individual application areas or on the technology itself.

Fluorescence in-situ hybridisation is a special type of ISH in which fluorescent markers are used to visualise the probes. It can be used for various purposes, such as the detection of gene amplifications or gene translocations. Please contact us if you require further information on the individual application areas or on the technology itself.

Chromogenic in-situ hybridisation is a special type of ISH in which a chromogenic signal detection method similar to IHC is employed to visualise the probes. It can be used for a wide range of purposes, such as the detection of oncogenic viruses. Please contact us if you require further information on the individual application areas or on the technology itself.