Multiplexing

The term «Spatial Biology» has quickly become an established concept among scientists around the world. 

«Spatial Biology» is a rapidly developing and dynamic field which uses the spatial structure, location, organisation and function of biological molecules in cells and tissues to understand how they interact with each other and control various biological processes. A further advantage of these multiplexing analyses is that several protein expression patterns can be compared with each other using only a single sample.

With our comprehensive selection of different multiplexing technologies, you can specifically investigate protein expression, localisation and interaction in your samples.

We can offer customised chromogenic and fluorescent staining for human and animal tissue. Our multiplex stains are performed fully automated on our Leica BOND RX immunostainer. This way, we guarantee consistent, reproducible stains of the highest quality and optimum preservation of the tissue morphology at the same time, even over several staining cycles.

Please contact us if you require further information on the individual multiplexing technologies. 

Chromogenic multiplex staining allows the visualisation of up to 6 chromogens on one tissue section and thus the detection of up to 6 different target structures within a single sample. The main advantage of chromogenic multiplex staining is that it produces a multi-colour slide which is permanently resistant to photobleaching.

Fluorescence multiplex staining has also emerged as a valuable tool for identifying and detecting complex protein interactions. We employ the OPAL™ multiplex method from Akoya Biosciences. OPAL™ is based on tyramide signal amplification (TSA) and is possible with any combination of antibodies, regardless of their species. Up to 7 different markers can be combined in a fluorescence multiplex stain.