Immunostaining
Immunostaining is an indispensable tool in immunology, molecular biology, basic research, drug development and pathology, enabling researchers to gain a better understanding of biological processes and disease mechanisms. Our comprehensive range of immunostaining techniques allows you to investigate protein expression, localisation and distribution in your samples.
We offer tailor-made staining using immunohistochemistry and immunofluorescence for human and animal tissue (for FFPE tissue and frozen sections) as well as staining of organoid cells, cytospins, smear preparations and samples of human skin equivalents.
All our immunostains are performed fully automated on our Leica BOND immunostainer. This way, we guarantee consistent, reproducible stains of the highest quality and optimum preservation of the tissue morphology at the same time.
Selection and optimisation of antibodies
Antibodies can often be used in multiple assays, however, they are not equally good in all assays. This applies in particular to immunostaining, especially when working with tissue samples that have been fixed with formalin and embedded in paraffin. Immunostaining is a complex procedure whose results are influenced by a variety of parameters. It is therefore important that such an assay is based on a solid foundation, which already begins with the selection of suitable antibodies. We would be pleased to support you with our many years of experience in the search for suitable markers and carry out optimisation on suitable positive and negative controls.
We can offer the following immunostaining techniques. Please contact us if you require further information on the individual staining techniques.